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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Insulin promotes the bone formation capability of human dental pulp stem cells through attenuating the IIS/PI3K/AKT/mTOR pathway axis
doi: 10.1186/s13287-024-03843-9
Figure Lengend Snippet: Primer sequences used for RT-qPCR
Article Snippet: Membranes were blocked with 5% non-fat powdered milk (catalog no. D8340, Solarbio) for 1 h. After washing the film with Tris-Glycine Running Buffer (catalog no. T1070, Solarbio), the membranes were probed with primary antibodies including rabbit anti-human glyceraldehyde-3-phosphate dehydrogenase (glyceraldehyde-3-phosphate dehydrogenase GAPDH) (37 kDa) polyclonal antibody (catalog no. AF0911, 1:1000, Affinity Biosciences), rabbit anti-human COL-1 (130–150 kDa) polyclonal antibody (catalog no. AF7001, 1:1000, Affinity Biosciences), rabbit anti-human RUNX2 (57 kDa) polyclonal antibody (catalog no. AF5186, 1:1000, Affinity Biosciences), rabbit anti-human ALP (35 kDa) polyclonal antibody (catalog no. DF12525, 1:1000, Affinity Biosciences), rabbit anti-human OCN (11 kDa) polyclonal antibody (catalog no. DF12303, 1:1000, Affinity Biosciences),
Techniques: